Department of Fishery Microbiology

Achievements

  • Under the DBT funded project, developed a highly efficient immunostimulant for shrimp. The  technology has been transferred to industry and the product  sold under the brand name "AQUASTIM”. 
  • Developed for the first  time in the country, a highly sensitive and specific diagnostic method based on  Polymerase Chain Reaction (PCR) for rapid detection of  Whitespot Syndrome Virus WSSV ) which is  affecting aquaculture industry in Asia. This technology has been  transferred to the industry on an  commercial basis. 
  • Played a central role in dissemination of PCR diagnostic techniques in aquaculture sector in India.  Having  developed PCR diagnostic, trained officers of Marine Products Export Development Authority (MPEDA )  of Ministry of Commerce, Government of India. Helped MPEDA, Government of Andhra Pradesh, Government of Orissa to set up PCR laboratories.
  • Help set up PCR laboratories in various shrimp hatcheries.
  • Developed a highly sensitive rapid PCR based method for detection of pathogenic strains of  Vibrio  parahaemolyticus in seafoods. Presently, in the process of  training MPEDA and Fish Control Officers in this technology. Filing of patent application in process.
  • For the first time developed PCR based detection method for toxic dinoflagellates and their cysts, which cause fish kills and shellfish poisoning.
  • First in the country to document Paralytic shellfish toxicity and characterize the toxins  by high performance  liquid chromatography (HPLC).

Technologies developed
Immunostimulant for shrimp health management (aquastim)
An immunostimulant (Aquasting) containing bacterin, lipopolysaccharides and yeast glucans has been developed. Laboratory studies indicate that administration of the immunostimulant by immersion or through feed stimulates the immune system of shrimp. In hatchery, use of immunostimulant has been shown to improve survival by over 20% 

Kit for rapid detection of Vibrio harveyi in hatcheries

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V. harveyi colonies

 

PCR detection of V. harveyi

A PCR based test for rapid detection of V. harveyi in hatchery water and P. monodon  larvae has been developed. This kit would be helpful for assessing the quality of P. monodon  larvae and for monitoring eggs, larvae and hatchery water for the presence of this serious pathogen.
PCR kit for detection of white spot syndrome virus, Monodon baculo viruses, Hepatopancreatic parvovirus, infections hypodermal, hematopoietic necrosis virus and laem singh virus have been developed.

 

 

Combi  kit  for  simultaneous  detection  of  white  spot  syndrome  virus (WSSV)  and monodon  baculovirus (MBV)
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The COMBI WSSV-MBV is a kit designed to detect very low levels of WSSV and MBV in shrimp by DNA amplification method in a single reaction.  The kit employs nested amplification which is 103-104 times more sensitive compared to normal one step Polymerase Chain Reaction (PCR). The method is simple and takes only 6 hrs from sample preparation to the results.
LUMINIL – Bacteriophage therapy

 Luminous bacterial disease is caused by Vibrio harveyi one of the greatest problems in shrimp hatcheries and farms. A biocontrol of the Vibrio is developed employing phage theraphy

 

 

 

 

 

 


Oligonucleotide probe for the detection and enumeration of Vibrio species in aquaculture systems

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Colony hybridization

 

Vibrio species are serious shrimp pathogens causing mortalities in hatcheries and grow out systems. Vibrio counts in hatchery systems and shrimp ponds are considered an indicator of the health of the systems, higher counts indicating poor health. A specific oligonucleotide probe for enumeration of Vibrios by colony hybridization after plating in non-selective agar medium such as Tryptone soya agar has been developed. The DNA probe can be used to detect and enumerate all Vibrio species on any non selective medium. This will facilitate growth metabolically injured Vibrios subjected to sanitizer treatment.

Development of recombinant protein vaccine against Aeromonas hydrophila
 

Aeromonas hydrophila causes motile aeromonad septicemia which results in great economic losses for the fish-farming industry. A recombinant vaccine developed against this pathogen has provided high protection (RPS ~ 65%) when challenged with  A.hydrophila.

Development of recombinant protein vaccine against Edwardsiella tarda

 

Edwardsiella tarda is responsible for ‘Edwardsiellosis’, a disease characterized by septicemia with extensive skin lesions and swelling in fish.  We have developed a recombinant vaccine with high protection (RPS ~70%) when challenged with E.tarda.

Rapid detection of Aeromonas hydrophila by PCR and dot blot

 

 

Aeromonas hydrophila has been predominantly involved in warm water fish disease. A  PCR based dot blot method for rapid detection of Aeromonas spp. targeting a common gene of all aeromonads  has been developed. 

Rapid detection of edwardsiella tarda by PCR

 

A  PCR for rapid detection of E. tarda targeting a common gene has been developed.